- Titel
- Vibrio natriegens as a superior host for the production of c-type cytochromes and difficult-to-express redox proteins
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:105-qucosa2-1013075
- Quellenangabe
- Scientific reports
Erscheinungsjahr: 2024
Jahrgang: 14
Seiten: 1-10
ISSN: 2045-2322
Artikelnummer: 6093 - Erstveröffentlichung
- 2024
- Abstract (EN)
- C-type cytochromes fulfil many essential roles in both aerobic and anaerobic respiration. Their characterization requires large quantities of protein which can be obtained through heterologous production. Heterologous production of c-type cytochromes in Escherichia coli is hindered since the ccmABCDEFGH genes necessary for incorporation of heme c are only expressed under anaerobic conditions. Different strategies were devised to bypass this obstacle, such as co-expressing the ccm genes from the pEC86 vector. However, co-expression methods restrict the choice of expression host and vector. Here we describe the first use of Vibrio natriegens Vmax X2 for the recombinant production of difficult-to-express redox proteins from the extreme acidophile Acidithiobacillus ferrooxidans CCM4253, including three c-type cytochromes. Co-expression of the ccm genes was not required to produce holo-c-type cytochromes in Vmax X2. E. coli T7 Express only produced holo-c-type cytochromes during co-expression of the ccm genes and was not able to produce the inner membrane cytochrome CycA. Additionally, Vmax X2 cell extracts contained higher portions of recombinant holo-proteins than T7 Express cell extracts. All redox proteins were translocated to the intended cell compartment in both hosts. In conclusion, V. natriegens represents a promising alternative for the production of c-type cytochromes and difficult-to-express redox proteins.
- Andere Ausgabe
- Vibrio natriegens as a superior host for the production of c-type cytochromes and difficult-to-express redox proteins
DOI: 10.1038/s41598-024-54097-7 - Freie Schlagwörter (DE)
- Publikationsfonds, Genexpression, Escherichia coli, Proteintransport, Cytochrom c, Acidithiobacillus ferrooxidans, Vibrio natriegens, Bakterien, Heterologe Genexpression, Rekombinantes Protein, Biochemie, Molekularbiologie, Gen, Redoxproteine, Holoprotein
- Freie Schlagwörter (EN)
- publishing fund
- Klassifikation (DDC)
- 572
- Normschlagwörter (GND)
- Genexpression
- Escherichia coli
- Membranproteine
- Proteintransport
- Cytochrom c
- Acidithiobacillus ferrooxidans
- Vibrio natriegens
- Proteine
- Bakterien
- Heterologe Genexpression
- Rekombinantes Protein
- Biochemie
- Molekularbiologie
- Gen
- Verlag
- Springer Nature, London
- Sonstige beteiligte Institution
- Technische Universität Bergakademie Freiberg, Freiberg
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:105-qucosa2-1013075
- Veröffentlichungsdatum Qucosa
- 07.01.2026
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0